重組人R-Spondin-1
重組人R-Spondin-1是一種25.6kDa的蛋白質(zhì)，由243個(gè)氨基酸殘基組成。隨著類器官領(lǐng)域的擴(kuò)大，R-Spondin 1被廣泛用于類器官細(xì)胞培養(yǎng)中，作為促進(jìn)3D類器官生長和存活的重要成分。R-Spondin-1（Rspo-1）屬于Wnt調(diào)節(jié)劑的（Rspo）家族。目前，該家族由四個(gè)結(jié)構(gòu)相關(guān)的分泌配體（Rspo 1-4）組成，所有配體都含有弗林樣結(jié)構(gòu)域和血小板反應(yīng)蛋白結(jié)構(gòu)域。Rspo-1在發(fā)育中的中樞神經(jīng)系統(tǒng)的某些區(qū)域以及腎上腺、卵巢、睪丸、甲狀腺和氣管中表達(dá)。Rspo可以以刺激Wnt/β-catenin信號(hào)通路的方式與Frizzled/LRP6受體復(fù)合物相互作用。
(純度) Purity
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
(內(nèi)毒素) Endotoxin
≤10 EU/mg by the LAL method.
(生物活性) Bioactivity
Measured by its ability to induce Topflash reporter bioactivity in CHO cells in the presence of 50 ng/mL wnt3a. The expected ED50 is <1.0 μg/mL.
(種屬) Species
Human
(表達(dá)系統(tǒng)) Expression System
CHO
(預(yù)測分子量大小) Mol. Weight
26.7 kDa
(表達(dá)區(qū)間) Expression Region
Ser21-Ala263
(標(biāo)簽) Tag Info
Tag free
(劑型) Formulation
Lyophilized
(溶液) Buffer
PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
(規(guī)格) Size
10μg/50μg/1mg
(復(fù)溶) Reconstitution
It is recommended to redissolve in sterile deionized water.
(運(yùn)輸) Shipping
The product is shipped with blue ice.
(存儲(chǔ)) Storage & Stability
36 months at-20°C to -80°C in lyophilized state;
6 months at -20°C to -80°C under sterile conditions after reconstitution;
7-10 days at 2°C to 8°C under sterile conditions after reconstitution;
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.?
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